A cloned antigen for serological diagnosis of Wuchereria bancrofti microfilaremia with daytime blood samples.
Identifieur interne : 00D175 ( Main/Exploration ); précédent : 00D174; suivant : 00D176A cloned antigen for serological diagnosis of Wuchereria bancrofti microfilaremia with daytime blood samples.
Auteurs : S. Dissanayake [États-Unis] ; M. Xu ; W F PiessensSource :
- Molecular and biochemical parasitology [ 0166-6851 ] ; 1992.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (sang), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Clonage moléculaire, Diéthylcarbamazine (usage thérapeutique), Données de séquences moléculaires, Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Filariose lymphatique (traitement médicamenteux), Filariose lymphatique (épidémiologie), Gènes d'helminthe (génétique), Humains, Immunoglobuline G (sang), Protéines d'helminthes (génétique), Protéines d'helminthes (immunologie), Protéines de fusion recombinantes (biosynthèse), Protéines de liaison au maltose, Protéines de transport (biosynthèse), Protéines de transport (génétique), Spécificité des anticorps, Sri Lanka, Séquence d'acides aminés, Séquence nucléotidique, Test ELISA, Wuchereria bancrofti (génétique), Wuchereria bancrofti (immunologie).
- MESH :
- biosynthèse : Protéines de fusion recombinantes, Protéines de transport.
- diagnostic : Filariose lymphatique.
- génétique : Antigènes d'helminthe, Gènes d'helminthe, Protéines d'helminthes, Protéines de transport, Wuchereria bancrofti.
- immunologie : Antigènes d'helminthe, Filariose lymphatique, Protéines d'helminthes, Wuchereria bancrofti.
- sang : Anticorps antihelminthe, Immunoglobuline G.
- traitement médicamenteux : Filariose lymphatique.
- usage thérapeutique : Diéthylcarbamazine.
- épidémiologie : Filariose lymphatique.
- Animaux, Clonage moléculaire, Données de séquences moléculaires, Humains, Protéines de liaison au maltose, Spécificité des anticorps, Sri Lanka, Séquence d'acides aminés, Séquence nucléotidique, Test ELISA.
- Wicri :
- geographic : Sri Lanka.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Helminth (blood), Antibody Specificity, Antigens, Helminth (genetics), Antigens, Helminth (immunology), Base Sequence, Carrier Proteins (biosynthesis), Carrier Proteins (genetics), Cloning, Molecular, Diethylcarbamazine (therapeutic use), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (drug therapy), Elephantiasis, Filarial (epidemiology), Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay, Genes, Helminth (genetics), Helminth Proteins (genetics), Helminth Proteins (immunology), Humans, Immunoglobulin G (blood), Maltose-Binding Proteins, Molecular Sequence Data, Recombinant Fusion Proteins (biosynthesis), Sri Lanka, Wuchereria bancrofti (genetics), Wuchereria bancrofti (immunology).
- MESH :
- chemical , biosynthesis : Carrier Proteins, Recombinant Fusion Proteins.
- chemical , blood : Antibodies, Helminth, Immunoglobulin G.
- chemical , genetics : Antigens, Helminth, Carrier Proteins, Helminth Proteins.
- chemical , immunology : Antigens, Helminth, Helminth Proteins.
- chemical , therapeutic use : Diethylcarbamazine.
- geographic : Sri Lanka.
- diagnosis : Elephantiasis, Filarial.
- drug therapy : Elephantiasis, Filarial.
- epidemiology : Elephantiasis, Filarial.
- genetics : Genes, Helminth, Wuchereria bancrofti.
- immunology : Elephantiasis, Filarial, Wuchereria bancrofti.
- Amino Acid Sequence, Animals, Antibody Specificity, Base Sequence, Cloning, Molecular, Enzyme-Linked Immunosorbent Assay, Humans, Maltose-Binding Proteins, Molecular Sequence Data.
Abstract
By differentially screening an adult Brugia malayi cDNA library with sera from microfilaremic and amicrofilaremic donors infected with Wuchereria bancrofti, we have identified a novel parasite antigen denoted SXP-1. Recombinant SXP-1 filarial antigen is preferentially recognized by sera from microfilaremic persons with bancroftian filariasis and from skin snip-positive patients with onchocerciasis. Antibodies to SXP-1 are restricted to the IgG4 subclass and gradually decline after treatment with diethylcarbamazine. These findings indicate that it may be possible to replace microscopic examination of night blood films with a serological test designed to detect antibodies to a mix of SXP-1 and other suitable antigens for the diagnosis of microfilaremia due to bancroftian filariasis.
PubMed: 1484551
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 005832
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- to stream PubMed, to step Checkpoint: 005832
- to stream Ncbi, to step Merge: 001861
- to stream Ncbi, to step Curation: 001861
- to stream Ncbi, to step Checkpoint: 001861
- to stream Main, to step Merge: 00DC70
- to stream Main, to step Curation: 00D175
Le document en format XML
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Dissanayake</name><affiliation wicri:level="2"><nlm:affiliation>Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Massachusetts</region></placeName><wicri:cityArea>Department of Tropical Public Health, Harvard School of Public Health, Boston</wicri:cityArea></affiliation></author><author><name sortKey="Xu, M" sort="Xu, M" uniqKey="Xu M" first="M" last="Xu">M. Xu</name></author><author><name sortKey="Piessens, W F" sort="Piessens, W F" uniqKey="Piessens W" first="W F" last="Piessens">W F Piessens</name></author></analytic><series><title level="j">Molecular and biochemical parasitology</title><idno type="ISSN">0166-6851</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Antibody Specificity</term><term>Antigens, Helminth (genetics)</term><term>Antigens, Helminth (immunology)</term><term>Base Sequence</term><term>Carrier Proteins (biosynthesis)</term><term>Carrier Proteins (genetics)</term><term>Cloning, Molecular</term><term>Diethylcarbamazine (therapeutic use)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (drug therapy)</term><term>Elephantiasis, Filarial (epidemiology)</term><term>Elephantiasis, Filarial (immunology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Genes, Helminth (genetics)</term><term>Helminth Proteins (genetics)</term><term>Helminth Proteins (immunology)</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Maltose-Binding Proteins</term><term>Molecular Sequence Data</term><term>Recombinant Fusion Proteins (biosynthesis)</term><term>Sri Lanka</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (sang)</term><term>Antigènes d'helminthe (génétique)</term><term>Antigènes d'helminthe (immunologie)</term><term>Clonage moléculaire</term><term>Diéthylcarbamazine (usage thérapeutique)</term><term>Données de séquences moléculaires</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (traitement médicamenteux)</term><term>Filariose lymphatique (épidémiologie)</term><term>Gènes d'helminthe (génétique)</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Protéines d'helminthes (génétique)</term><term>Protéines d'helminthes (immunologie)</term><term>Protéines de fusion recombinantes (biosynthèse)</term><term>Protéines de liaison au maltose</term><term>Protéines de transport (biosynthèse)</term><term>Protéines de transport (génétique)</term><term>Spécificité des anticorps</term><term>Sri Lanka</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Test ELISA</term><term>Wuchereria bancrofti (génétique)</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Carrier Proteins</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, 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antihelminthe</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Diéthylcarbamazine</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibody Specificity</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Maltose-Binding Proteins</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Protéines de liaison au maltose</term><term>Spécificité des anticorps</term><term>Sri Lanka</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Test ELISA</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Sri Lanka</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">By differentially screening an adult Brugia malayi cDNA library with sera from microfilaremic and amicrofilaremic donors infected with Wuchereria bancrofti, we have identified a novel parasite antigen denoted SXP-1. Recombinant SXP-1 filarial antigen is preferentially recognized by sera from microfilaremic persons with bancroftian filariasis and from skin snip-positive patients with onchocerciasis. Antibodies to SXP-1 are restricted to the IgG4 subclass and gradually decline after treatment with diethylcarbamazine. These findings indicate that it may be possible to replace microscopic examination of night blood films with a serological test designed to detect antibodies to a mix of SXP-1 and other suitable antigens for the diagnosis of microfilaremia due to bancroftian filariasis.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><noCountry><name sortKey="Piessens, W F" sort="Piessens, W F" uniqKey="Piessens W" first="W F" last="Piessens">W F Piessens</name><name sortKey="Xu, M" sort="Xu, M" uniqKey="Xu M" first="M" last="Xu">M. Xu</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Dissanayake, S" sort="Dissanayake, S" uniqKey="Dissanayake S" first="S" last="Dissanayake">S. Dissanayake</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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